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US6773564B1
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Glucose sensor
Assignee: PHC Holdings Corp
•
Filing: 1999-09-28
•
Granted: 2004-08-10
•
Expires: 2019-09-28
Abstract
Abstract The present invention provides a high performance glucose sensor which can demonstrate high stability against preservation and produces only a low blank value. The glucose sensor comprises an electrically insulating base plate, an electrode system formed on the base plate, and a reaction layer which is formed in contact with or in the vicinity of the electrode system and contains at least a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, the reaction layer further containing an additive such as phthalic acid.
Inventors
- 👤 Keiko Yugawa
- 👤 Toshihiko Yoshioka
- 👤 Shiro Nankai
- 👤 Junko Iwata
- 👤 Shoji Miyazaki
- 👤 Hideyuki Baba
- 👤 Seiji Takeshima
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 23:54
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/001; C12Q1/005; C12Q1/006
Claims Analysis
Total Claims: 89
Claim 1
Independent Claim
64 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system, wherein said reaction layer contains phthalic acid or a phthalate, and a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone.
Claim 2
Dependent Claim
64 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system, wherein said reaction layer contains phthalic acid or a phthalate, and a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone.
Claim 3
Dependent Claim
44 words
The glucose sensor in accordance with claim 1 , wherein said reaction layer further contains a stabilizer, said stabilizer having a function of retaining the activity of the enzyme and the long-term preservation of said sensor and decreasing the blank value of said sensor.
Claim 4
Dependent Claim
35 words
The glucose sensor in accordance with claim 2 , wherein said stabilizer is a metal salt, an organic acid, a protein, an amide acid, a sugar or a derivative thereof, a surfactant, or ammonium sulfate.
Claim 5
Dependent Claim
32 words
The glucose sensor in accordance with claim 3 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, a strontium salt and a manganese salt.
Claim 6
Dependent Claim
21 words
The glucose sensor in accordance with claim 4 , wherein said metal salt is a sulfate, a nitrate or a halide.
Claim 7
Dependent Claim
17 words
The glucose sensor in accordance with claim 4 , wherein said calcium salt is CaCl 2 .
Claim 8
Dependent Claim
35 words
The glucose sensor in accordance with claim 3 , wherein said stabilizer is an organic acid selected from the group consisting of α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 9
Dependent Claim
28 words
The glucose sensor in accordance with claim 3 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 10
Dependent Claim
50 words
The glucose sensor in accordance with claim 3 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide and a derivative thereof, an oligosaccharide and a derivative thereof and a polysaccharide and a derivative thereof.
Claim 11
Dependent Claim
37 words
The glucose sensor in accordance with claim 9 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol, glucosamine and deoxyglucose, or a derivative thereof.
Claim 12
Dependent Claim
30 words
The glucose sensor in accordance with claim 9 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 13
Dependent Claim
33 words
The glucose sensor in accordance with claim 9 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 14
Dependent Claim
31 words
The glucose sensor in accordance with claim 9 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
Claim 15
Dependent Claim
28 words
The glucose sensor in accordance with claim 3 , said stabilizer is an amido acid selected from the group consisting of lysine, histidine, glutamic acid, glycylglycine and polylysine.
Claim 16
Dependent Claim
16 words
The glucose sensor in accordance with claim 3 , wherein said stabilizer is a non-ionic surfactant.
Claim 17
Dependent Claim
55 words
The glucose sensor in accordance with claim 1 , wherein said reaction layer further contains maleic acid, a maleate, succinic acid, a succinate, triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino) ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris (hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole or collidine.
Claim 18
Independent Claim
65 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, wherein at least one additive is added to glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, said additive being selected from the group consisting of phthalic acid, a phthalate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 19
Independent Claim
65 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, wherein at least one additive is added to glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, said additive being selected from the group consisting of phthalic acid, a phthalate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 20
Independent Claim
43 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, said glucose sensors each having a reaction layer comprising a glucose-dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, wherein phthalic acid or a phthalate is added to said glucose-dehydrogenase whose coenzyme is pyrrolo-quinoline quinone.
Claim 21
Independent Claim
43 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, said glucose sensors each having a reaction layer comprising a glucose-dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, wherein phthalic acid or a phthalate is added to said glucose-dehydrogenase whose coenzyme is pyrrolo-quinoline quinone.
Claim 22
Dependent Claim
57 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 18 , wherein a stabilizer is added to said glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, said stabilizer having a function of retaining the activity of enzyme and the long-term preservation of said sensor and decreasing the blank value of said sensor.
Claim 23
Dependent Claim
43 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 19 , wherein said stabilizer is a metal salt, an organic acid, a protein, an amino acid, a sugar or a derivative thereof, a surfactant, or ammonium sulfate.
Claim 24
Dependent Claim
40 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, a strontium salt and a manganese salt.
Claim 25
Dependent Claim
29 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 21 , wherein said metal salt is a sulfate, a nitrate or a halide.
Claim 26
Dependent Claim
25 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 21 , wherein said calcium salt is CaCl 2 .
Claim 27
Dependent Claim
43 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is an organic acid selected from the group consisting of α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 28
Dependent Claim
36 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 29
Dependent Claim
58 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide and a derivative thereof, an oligosaccharide and a derivative thereof, and a polysaccharide and a derivative thereof.
Claim 30
Dependent Claim
45 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 26 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol, glucosamine and deoxyglucose, or a derivative thereof.
Claim 31
Dependent Claim
38 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 26 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 32
Dependent Claim
41 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 26 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 33
Dependent Claim
39 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 26 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
Claim 34
Dependent Claim
37 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is an amino acid selected from the group consisting of lysine, histidine, glutamic acid, glycylglycine and polylysine.
Claim 35
Dependent Claim
24 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 20 , wherein said stabilizer is a non-ionic surfactant.
Claim 36
Dependent Claim
65 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with any of claim 18 to 32 , wherein said reaction layer further contains maleic acid, a maleate, succinic acid, a succinate, triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole or collidine.
Claim 37
Dependent Claim
60 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, and at lease one additive selected from the group consisting of phthalic acid, a phthalate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, (N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 38
Dependent Claim
60 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, and at lease one additive selected from the group consisting of phthalic acid, a phthalate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, (N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 39
Dependent Claim
25 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, and phthalic acid or a phthalate.
Claim 40
Dependent Claim
25 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, and phthalic acid or a phthalate.
Claim 41
Dependent Claim
48 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 35 , said composition further containing a stabilizer, said stabilizer having a function of retaining the activity of the enzyme and the long-term preservation of said sensor and decreasing the blank value of said sensor.
Claim 42
Dependent Claim
41 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 36 , wherein said stabilizer is a metal salt, an organic acid, a protein, an amino acid, a sugar or a derivative thereof, a surfactant, or ammonium sulfate.
Claim 43
Dependent Claim
38 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, a strontium salt and a manganese salt.
Claim 44
Dependent Claim
27 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 38 , wherein said metal salt is a sulfate, a nitrate or a halide.
Claim 45
Dependent Claim
23 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 38 , wherein said calcium salt is CaCl 2 .
Claim 46
Dependent Claim
42 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , wherein said stabilizer is an organic acid selected from the group consisting of a α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 47
Dependent Claim
34 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 48
Dependent Claim
56 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide and a derivative thereof, an oligosaccharide and a derivative thereof, and a polysaccharide and a derivative thereof.
Claim 49
Dependent Claim
43 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 43 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol glucosamine and deoxyglucose, or a derivative thereof.
Claim 50
Dependent Claim
36 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 43 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 51
Dependent Claim
39 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 43 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 52
Dependent Claim
37 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 43 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
Claim 53
Dependent Claim
34 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , said stabilizer is an amino acid selected from the group consisting of lysine, histidine, glutamic acid, glycylglycine and polylysine.
Claim 54
Dependent Claim
22 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 37 , wherein said stabilizer is a non-ionic surfactant.
Claim 55
Dependent Claim
62 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with any of claims 35 to 49 , wherein said composition further contains maleic acid, a maleate, succinic acid, a succinate, triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole or collidine.
Claim 56
Dependent Claim
97 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system wherein said reaction layer contains: a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and an additive selected from the group consisting of triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 57
Dependent Claim
97 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system wherein said reaction layer contains: a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and an additive selected from the group consisting of triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 58
Dependent Claim
37 words
The glucose sensor in accordance with claim 51 , further comprising a at least one stabilizer selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof.
Claim 59
Dependent Claim
33 words
The glucose sensor in accordance with claim 51 , wherein the response of the sensor to glucose within the concentration range of 0 to 500 mg/dl is substantially linear after long term preservation.
Claim 60
Independent Claim
91 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, wherein a stabilizer and a buffer are added to glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, said stabilizer being selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof, and said buffer being selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 61
Independent Claim
91 words
A method for stabilizing glucose dehydrogenase for use in glucose sensors, wherein a stabilizer and a buffer are added to glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone, said stabilizer being selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof, and said buffer being selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 62
Dependent Claim
43 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 54 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, CaCl 2 , a strontium salt and a manganese salt.
Claim 63
Dependent Claim
43 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 54 , wherein said stabilizer is an organic acid selected from the group consisting of α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 64
Dependent Claim
36 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 54 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 65
Dependent Claim
58 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 54 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide and a derivative thereof, an oligosaccharide and a derivative thereof, and a polysaccharide and a derivative thereof.
Claim 66
Dependent Claim
45 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 58 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol, glucosamine and deoxyglucose, or a derivative thereof.
Claim 67
Dependent Claim
38 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 58 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 68
Dependent Claim
41 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 58 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 69
Dependent Claim
39 words
The method for stabilizing glucose dehydrogenase for use in glucose sensors in accordance with claim 58 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
Claim 70
Dependent Claim
84 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing: at least one stabilizer selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof; a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and a buffer selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 71
Dependent Claim
84 words
A glucose dehydrogenase composition for use in glucose sensors, said composition containing: at least one stabilizer selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof; a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and a buffer selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 72
Dependent Claim
41 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 63 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, CaCl 2 , a strontium salt and a manganese salt.
Claim 73
Dependent Claim
41 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 63 , wherein said stabilizer is an organic acid selected from the group consisting of α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 74
Dependent Claim
34 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 63 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 75
Dependent Claim
56 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 63 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide and a derivative thereof, an oligosaccharide and a derivative thereof, and a polysaccharide and a derivative thereof.
Claim 76
Dependent Claim
43 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 67 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol, glucosamine and deoxyglucose, or a derivative thereof.
Claim 77
Dependent Claim
36 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 67 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 78
Dependent Claim
39 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 67 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 79
Dependent Claim
37 words
The glucose dehydrogenase composition for use in glucose sensors in accordance with claim 67 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
Claim 80
Dependent Claim
126 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system wherein said reaction layer contains: at least one stabilizer selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof; a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and an additive selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 81
Dependent Claim
126 words
A glucose sensor comprising an electrically insulating base plate, an electrode system including at least a working electrode and a counter electrode formed on said base plate, and a reaction layer which is formed in contact with or in the vicinity of said electrode system wherein said reaction layer contains: at least one stabilizer selected from the group consisting of a metal salt, an organic acid, a protein, and a sugar and a derivative thereof; a glucose dehydrogenase whose coenzyme is pyrrolo-quinoline quinone; and an additive selected from the group consisting of maleic acid, a maleate, triethanol amine, a triethanol amine salt, citric acid, a citrate, dimethyl glutaric acid, 2-(N-morpholino)ethane sulfonic acid, a 2-(N-morpholino)ethane sulfonate, tris(hydroxymethyl)glycine, a tris(hydroxymethyl)glycine salt, tris(hydroxymethyl)aminomethane, a tris(hydroxymethyl)aminomethane salt, imidazole and collidine.
Claim 82
Dependent Claim
35 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a metal salt selected from the group consisting of a calcium salt, CaCl 2 , a strontium salt and a manganese salt.
Claim 83
Dependent Claim
35 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is an organic acid selected from the group consisting of α-ketoglutaric acid, malic acid, fumaric acid, gluconic acid, cholic acid and deoxycholic acid.
Claim 84
Dependent Claim
28 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a protein selected from the group consisting of bovine serum albumin, egg albumin and gelatin.
Claim 85
Dependent Claim
50 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a sugar or a derivative thereof selected from the group consisting of a monosaccharide and a derivative thereof, a disaccharide, and a derivative thereof, an oligosaccharide and a derivative thereof, and a polysaccharide and a derivative thereof.
Claim 86
Dependent Claim
37 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a monosaccharide selected from the group consisting of glucose, fructose, galactose, mannose, xylose, inositol, monnitol, sorbitol, ribitol, glucosamine and deoxyglucose, or a derivative thereof.
Claim 87
Dependent Claim
30 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a disaccharide selected from the group consisting of sucrose, lactose, maltose and trehalose, or a derivative thereof.
Claim 88
Dependent Claim
33 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is an oligosaccharide selected from the group consisting of malt triose, maltosyl cyclodextrin, α-cyclodextrin, β-cyclodextrin and γ-cyclodextrin, or a derivative thereof.
Claim 89
Dependent Claim
31 words
The glucose sensor in accordance with claim 72 , wherein said stabilizer is a polysaccharide selected from the group consisting of dextrin, amylose, glycogen, inulin and Ficoll, or a derivative thereof.
References Cited (Backward Citations)
US4545382A
Cited
US4711245A
Cited
US5820551A
Cited
US5682884A
Cited
US5334508A
Cited
EP0357027A2
Cited
US5466575A
Cited
US5554339A
Cited
US5378628A
Cited
US5804047A
Cited
US5424204A
Cited
US5762770A
Cited
JPH09140378A
Cited
US6025203A
Cited
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2007-08-10 | 2008-02-09 | 2008-08-10 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2011-08-10 | 2012-02-09 | 2012-08-10 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2015-08-10 | 2016-02-09 | 2016-08-10 | $7700.00 | Window Closed / Lapsed |