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US5451507A
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Method for immunochromatographic analysis
Assignee: Siemens Healthcare Diagnostics GmbH Germany
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Filing: 1994-05-10
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Granted: 1995-09-19
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Expires: 2014-05-10
Abstract
Abstract Methods are disclosed for conducting assays. One such method comprises providing in combination a first bibulous member zone ("first zone") and a liquid medium containing a component. The first zone has non-diffusively bound thereto a reagent interreactive with the component. Conditions are selected wherein the liquid medium and at least a portion of the component contained therein traverse all of the first zone and migrate by capillary migration into a second bibulous member zone ("second zone"). The second zone is of a different composition than the first zone and is incapable of specifically binding the component except when an analyte is to be detected and the method further includes causing a reagent to become bound to the first bibulous member zone in relation to the amount of analyte present. The distance the component has migrated into the second zone or the difference in the distances the medium and the component have migrated into the second zone is determined, the distance or the difference being related to the amount of the component in the liquid medium or the amount of the reagent.
Inventors
- 👤 Carl N. Skold
- 👤 Armen B. Shanafelt
- 👤 Vartan Ghazarossian
- 👤 Edwin F. Ullman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-06 12:41
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/54366; G01N33/54386; G01N33/54387; G01N33/54388; Y; Y10; Y10S; Y10S435/00; Y10S435/805; Y10S435/97; Y10S435/975; Y10T; Y10T436/00; Y10T436/19
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
190 words
A method for conducting an assay for the determination of an analyte, a component, or a reagent, which method comprises: providing in combination (1) a first bibulous member zone ("first zone") having a reagent non-diffusively bound thereto with the proviso that when an analyte is to be detected, said reagent becomes bound to the first zone in relation to the amount of analyte present, and (2) a liquid medium containing a component that is interreactive with said reagent, under conditions wherein said liquid medium and at least a portion of said component contained therein traverse all of said first zone and migrate by capillary migration into a second bibulous member zone ("second zone"), of different composition than said first zone and incapable of specifically binding said component except when an analyte is to be detected; and determining the distance said component has migrated into said second zone or the difference in the distances said medium and said component have migrated into said second zone, said distance or said difference being related to the amount of said analyte or said component in said liquid medium or the amount of said reagent.
Claim 2
Independent Claim
190 words
A method for conducting an assay for the determination of an analyte, a component, or a reagent, which method comprises: providing in combination (1) a first bibulous member zone ("first zone") having a reagent non-diffusively bound thereto with the proviso that when an analyte is to be detected, said reagent becomes bound to the first zone in relation to the amount of analyte present, and (2) a liquid medium containing a component that is interreactive with said reagent, under conditions wherein said liquid medium and at least a portion of said component contained therein traverse all of said first zone and migrate by capillary migration into a second bibulous member zone ("second zone"), of different composition than said first zone and incapable of specifically binding said component except when an analyte is to be detected; and determining the distance said component has migrated into said second zone or the difference in the distances said medium and said component have migrated into said second zone, said distance or said difference being related to the amount of said analyte or said component in said liquid medium or the amount of said reagent.
Claim 3
Dependent Claim
53 words
The method of claim 1 wherein prior to providing said combination said reagent is caused to non-diffusively bind to said first zone by contacting said first zone with a solution containing said reagent, and said difference or said distance is related to the amount of said reagent that binds to said first zone.
Claim 4
Dependent Claim
25 words
The method of claim 2 wherein said amount of said reagent is related to the amount of an analyte in said solution of said reagent.
Claim 5
Dependent Claim
20 words
The method of claim 1 wherein said first zone and said second zone are integral to a single bibulous member.
Claim 6
Dependent Claim
50 words
The method of claim 1 wherein said first zone is integral to a first bibulous member and said second zone is integral to a second bibulous member and said first zone is brought into liquid receiving relationship with said second zone during said assay and prior to providing said combination.
Claim 7
Dependent Claim
20 words
The method of claim 1 wherein said component and said reagent are each a member of a specific binding pair.
Claim 8
Dependent Claim
14 words
The method of claim 1 wherein said component and said reagent are chemically interreactive.
Claim 9
Dependent Claim
22 words
The method of claim 1 wherein said determining the distance said component has migrated into said second zone involves an oxidation-reduction reaction.
Claim 10
Dependent Claim
22 words
The method of claim 1 wherein said component is an enzyme labeled ligand analog and said reagent is antibody for said ligand.
Claim 11
Dependent Claim
24 words
The method of claim 1 wherein the difference in the distances said medium and said component have migrated into said second zone is determined.
References Cited (Backward Citations)
US3011874A
Cited
US3654090A
Cited
US3654090B1
Cited
US3646346A
Cited
US3791932A
Cited
US3817837A
Cited
US3966879A
Cited
US3996345A
Cited
US4062733A
Cited
US4168146A
Cited
US4104029A
Cited
US3990850A
Cited
US4067959A
Cited
US4094647A
Cited
US4120945A
Cited
US4055394A
Cited
US4108729A
Cited
US4160645A
Cited
US4160008A
Cited
US4220450A
Cited
US4233402A
Cited
US4438067A
Cited
US4298688A
Cited
US4189304A
Cited
US4287300A
Cited
US4327073A
Cited
US4366241A
Cited
US4366241B1
Cited
US4425438A
Cited
US4384958A
Cited
US4435504A
Cited
US4434236A
Cited
US4703003A
Cited
US4551426A
Cited
US4826759A
Cited
US4861711A
Cited
WO1986004683A1
Cited
US4744952A
Cited
US4868108A
Cited
US4973549A
Cited
US4883764A
Cited
EP0314499A1
Cited
US5334513A
Cited
US4999287A
Cited
US4959324A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1998-09-18 | 1999-03-20 | 1999-09-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2002-09-18 | 2003-03-20 | 2003-09-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2006-09-18 | 2007-03-20 | 2007-09-19 | $7700.00 | Window Closed / Lapsed |