US6087182A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Reagentless analysis of biological samples
Assignee: Abbott Laboratories
•
Filing: 1998-08-27
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Granted: 2000-07-11
•
Expires: 2018-08-27
Abstract
Abstract Apparatus and method for determining at least one parameter, e. g., concentration, of at least one analyte, e. g., urea, of a biological sample, e.g, urine. A biological sample particularly suitable for the apparatus and method of this invention is urine. In general, spectroscopic measurements can be used to quantify the concentrations of one or more analytes in a biological sample. In order to obtain concentration values of certain analytes, such as hemoglobin and bilirubin, visible light absorption spectroscopy can be used. In order to obtain concentration values of other analytes, such as urea, creatinine, glucose, ketones, and protein, infrared light absorption spectroscopy can be used. The apparatus and method of this invention utilize one or more mathematical techniques to improve the accuracy of measurement of parameters of analytes in a biological sample. The invention also provides an apparatus and method for measuring the refractive index of a sample of biological fluid while making spectroscopic measurements substantially simultaneously.
Inventors
- 👤 Tzyy-Wen Jeng
- 👤 Larry L. McDowell
- 👤 Joseph Larry Pezzaniti
- 👤 Gary M. Oosta
- 👤 Eric B. Shain
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 19:42
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/17; G01N21/41; G01N21/4133; G01N21/01; G01N21/03; G01N21/05; G01N21/25; G01N21/27; G01N21/274; Y; Y10; Y10T; Y10T436/00; Y10T436/14; Y10T436/142222; Y10T436/143333; Y10T436/144444; Y10T436/145555; Y10T436/146666; Y10T436/147777; Y10T436/17; Y10T436/171538; Y10T436/20; Y10T436/200833
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
329 words
A method for determining concentration of at least one analyte of interest in a liquid biological sample from an individual by means of spectroscopic analysis, said method comprising the steps of: (a) identifying at least one analyte that is a major component of said liquid biological sample, said at least one analyte accounting for significant variations with respect to a plurality of spectra of liquid biological samples from a plurality of donors of said liquid biological samples; (b) measuring a spectrum for each of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples; (c) calculating a model spectrum for each of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples by mathematically fitting spectra of said analytes of said at least one identified analyte to each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples; (d) calculating a residual spectrum havinq a non-zero value for each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples by subtracting each value of said model spectrum from each value of each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples that corresponds to said model spectrum; (e) repeating steps (a), (b), (c), and (d) at least one time by introducing at least one additional analyte to said model spectrum until said calculated residual spectra are substantially constant from one liquid biological sample to another liquid biological sample of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples; (f) determining a set of calibration parameters from said model spectra, said set of calibration parameters accounting for effects of said substantially constant residual spectra; and (g) using said calibration parameters to determine concentration of an analyte of interest in said liquid biological sample of said individual.
Claim 2
Independent Claim
329 words
A method for determining concentration of at least one analyte of interest in a liquid biological sample from an individual by means of spectroscopic analysis, said method comprising the steps of: (a) identifying at least one analyte that is a major component of said liquid biological sample, said at least one analyte accounting for significant variations with respect to a plurality of spectra of liquid biological samples from a plurality of donors of said liquid biological samples; (b) measuring a spectrum for each of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples; (c) calculating a model spectrum for each of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples by mathematically fitting spectra of said analytes of said at least one identified analyte to each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples; (d) calculating a residual spectrum havinq a non-zero value for each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples by subtracting each value of said model spectrum from each value of each spectrum of each of said liquid biological samples from said plurality of donors of said liquid biological samples that corresponds to said model spectrum; (e) repeating steps (a), (b), (c), and (d) at least one time by introducing at least one additional analyte to said model spectrum until said calculated residual spectra are substantially constant from one liquid biological sample to another liquid biological sample of said plurality of liquid biological samples from said plurality of donors of said liquid biological samples; (f) determining a set of calibration parameters from said model spectra, said set of calibration parameters accounting for effects of said substantially constant residual spectra; and (g) using said calibration parameters to determine concentration of an analyte of interest in said liquid biological sample of said individual.
Claim 3
Dependent Claim
35 words
The method of claim 1, wherein said biological sample is selected from the group consisting of urine, whole blood, plasma, serum, sputum, saliva, sweat, interstitial fluid, cerebral spinal fluid, and dialysate obtained in kidney dialysis.
Claim 4
Dependent Claim
10 words
The method claim 1, wherein said biological sample is urine.
Claim 5
Dependent Claim
26 words
The method of claim 3, wherein said at least one analyte of interest is selected from the group consisting of urea, creatinine, protein, glucose, and ketones.
Claim 6
Dependent Claim
23 words
The method of claim 3, wherein said at least one analyte of interest is selected from the group consisting of hemoglobin and bilirubin.
Claim 7
Dependent Claim
39 words
The method of claim 1, wherein said spectroscopic analysis is selected from the group consisting of ultraviolet absorption spectroscopy, visible absorption spectroscopy, infrared absorption spectroscopy, ultraviolet scattering spectroscopy, visible scattering spectroscopy, infrared scattering spectroscopy, fluorescence spectroscopy, and Raman spectroscopy.
Claim 8
Dependent Claim
12 words
The method of claim 1, wherein said spectroscopic analysis is filter photometry.
Claim 9
Dependent Claim
12 words
The method of claim 1, wherein said spectroscopic analysis is derivative spectroscopy.
Claim 10
Dependent Claim
14 words
The method of claim 1, wherein said spectroscopic analysis utilizes measurerilent of transmitted light.
Claim 11
Dependent Claim
14 words
The method of claim 1, wherein said spectroscopic analysis utilizes measurement of reflected light.
Claim 12
Dependent Claim
30 words
The method of claim 1, further including the step of employing at least one measurement selected from the following additional measurements: light scattering, impedance, pH, ion, temperature, and refractive index.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 2003-07-11 | 2004-01-10 | 2004-07-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 2007-07-11 | 2008-01-10 | 2008-07-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 2011-07-11 | 2012-01-10 | 2012-07-11 | $7700.00 | Window Closed / Lapsed |